WebMonarch PCR & DNA Cleanup Kit (5 μg) Monarch DNA Cleanup Binding Buffer. Monarch DNA Wash Buffer. Monarch DNA Elution Buffer. Monarch DNA Cleanup Columns (5 μg) RNAクリーンアップ. Monarch RNA Cleanup Kit (50 μg) Monarch RNA Cleanup Binding Buffer. Monarch RNA Cleanup Wash Buffer. WebThe Monarch PCR & DNA Cleanup Kit protocol can be modified to enable the purification of ssDNA, oligonucleotides, and other small DNA fragments. The Oligonucleotide …

HiScribe T7 High Yield RNA Synthesis Kit E2040 manual

Webreactions up to 16 hours (overnight) or using up to 2 μg of template will help to achieve maximum yield. Alternatively, clean up the DNA template using a spin column based … WebFor 50-prep kit add 24 ml the ethanol to 6 per starting Monarch Plasmid Wash Buffer 2 For 250-prep kit add 120 ml of distillable to 30 ml of Monarch Plasma Wash Buffer 2 Always keep all buffer bottles tightly closed as none actively in use. Logs: All centrifugation ladder should be carried out under 16,000 x g (~13,000 RPM). r banks open today

HiScribe T7 High Yield RNA Synthesis Kit E2040 manual

WebThe Monarch RNA Cleanup Kit (500 µg) reliably purifies up to 500 μg of concentrated, high-quality RNA (> 25 nt) from enzymatic reactions and in vitro transcription (IVT) … WebReactions are typically 25 µl but can be scaled up as needed. Reactions should be assembled in nuclease -free microfuge tubes or PCR tubes. Standard RNA Synthesis ... Webrecommend the Monarch® PCR and DNA Cleanup Kit (5 µg, NEB #T1030S). For long term storage at –20°C, store DNA in 10 mM Tris (pH 8.5), 1 mM EDTA (TE) or short-term storage in 10 mM Tris (pH 8.5), 0.1 mM EDTA (modified TE). EDTA at these levels will not significantly lower the 10 mM MgCl 2 rbank texas cd rates