WebFoam cell formation assay. The treated cells were soaked in 4% paraformaldehyde for 0.5 h, infiltrated for 5 min with 60% isopropanol and then incubated for 1 h with Oil Red O staining solution. A light microscopy was performed to observe the cells, and ImageJ software was used to calculate the percentage of intracellular lipid droplet area ... Foam cells are formed when circulating monocyte-derived cells are recruited to the atherosclerotic lesion site or fat deposits in the blood vessel walls. Recruitment is facilitated by the molecules P-selectin and E-selectin , intercellular adhesion molecule 1 ( ICAM-1 ) and vascular cell adhesion … See more Foam cells, also called lipid-laden macrophages, are a type of cell that contain cholesterol. These can form a plaque that can lead to atherosclerosis and trigger heart attacks and stroke. Foam cells are fat … See more Foam cell formation is triggered by a number of factors including the uncontrolled uptake of modified low density lipoproteins (LDL), the upregulation of cholesterol esterification and the impairment of mechanisms associated with cholesterol … See more Foam cell degradation or more specifically the breakdown of esterified cholesterols, is facilitated by a number of efflux receptors and pathways. Esterified cholesterol from cytoplasmic liquid … See more Foam cells may form around leaked silicone from breast implants. Lipid-laden alveolar macrophages, also known as pulmonary foam cells, are seen in bronchoalveolar lavage specimens in some respiratory diseases. See more Low-density lipoprotein (LDL) cholesterol (LDL-C — also known as “bad” cholesterol) and particularly modified forms of LDL cholesterol such as oxidized, glycated, or acetylated LDL, is contained by a foam cell - a marker of atherosclerosis. … See more Foamy macrophages are also found in diseases caused by pathogens that persist in the body, such as Chlamydia, Toxoplasma, or Mycobacterium tuberculosis See more

Pathogenesis of atherosclerosis: A multifactorial process - PMC

WebJun 24, 2024 · Background Foam cells (FCs) play crucial roles in the process of all stages of atherosclerosis. Smooth muscle cells (SMCs) and macrophages are the major sources of FCs. This study aimed to identify … Web2 days ago · Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2024 Published by Elsevier B.V. 1 Silica nanoparticles promoted pro-inflammatory macrophage and foam cell transformation via ROS/PPARγ/NF-κB signaling Caixia Guo … greenacre farm camping

Foam Cell - an overview ScienceDirect Topics

WebMacrophage foam cell formation represents one of the earliest stages of lesion development, ... Extracellular matrix glycoproteins within the intima contribute to this process by retaining apoB-containing lipoproteins and by modulating the activity of enzymes, including secretory phospholipase A2 group (PLA2G2A), PLA2G5, PLA2G7 … WebSep 23, 2013 · Uncontrolled uptake of oxidized low-density lipoprotein (ox-LDL), excessive cholesterol esterification and/or impaired cholesterol release result in accumulation of cholesterol ester (CE) stored as cytoplasmic lipid droplets and subsequently trigger the formation of foam cells. WebJul 30, 2016 · During the process of foam cell formation, co-culturing THP-M with oxLDL for 6 h in the presence of autophagy activator rapamycin (80 ug/mL) markedly decreased intracellular lipid content, while the autophagy inhibitor 3-MA (10 mM) considerably increased the intracellular lipid content (upper panel in Fig. 3a, b ). greenacre family dentist