Cy5 thermo

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August undefined, 2024

WebCy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the … WebImmunofluorescence analysis of Goat anti-Rat IgG (H+L) Secondary Antibody, Cy5® was performed using A549 cells stained with alpha Tubulin (YL1/2) Rat Monoclonal Antibody (Product # MA1-80017). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour …

7500 Real Time PCR Systems Spectral Calibration Kit II

WebMar 28, 2024 · The absorbance was recorded at 450 nm by spectrophotometer (Thermo Fisher, USA). And the viability of cells was calculated by comparing with control group (only fresh medium) and the experiment was repeated in triplicates. ... Cellular colocalization of Cy5-CpG 1826 and PDA NPs. RAW264.7 cells were initially cultured in 6- well plate with … WebOur labeling reagents enable researchers to create their own labeled biomolecule for use in immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling, and cytochemistry applications as well as for probing biological structure, function, and … flat top bbq built in

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WebCy5.5 dye. information in the instrument touchscreen before you perform the. Cy5.5 dye. calibration. 1. Load the plate. 2. In the home screen, touch . Settings. 4. Maintenance … WebThermo Fisher cy5 streptavidin Cy5 Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS … WebCatalog number: A30107. Alexa Fluor Plus 647 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, Cy5/deep red fluorescent Alexa Fluor Plus 647 dye. For the best possible sensitivity and specificity, Alexa Fluor Plus 647 Phalloidin is synthesized to maximize the units of Alexa Fluor ... flat top beach

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EVOS Light Cube Selection Guide Thermo Fisher Scientific - US

WebEach of the Alexa Fluor dyes are listed in Table 1 and described in detail in Alexa Fluor Dyes Spanning the Visible and Infrared Spectrum—Section 1.3 and in the accompanying Section 1.3 data table. Figure 1. Emission spectra for the Alexa Fluor dye series. Table 1. Spectral properties of Molecular Probes Alexa Fluor dyes. WebApr 10, 2024 · The polyA tail was 120 bp in length. Synthesized RNA was then purified and quantified by Nanodrop (Thermo Scientific). After purification, mRNA was resuspended in RNase-free water for use. ... a membrane cutoff of 30 kDa at 3000 g for 15 min. Fluorescent LNP/cLNP were prepared via addition of 0.2 mol% DSPE-PEG-Cy5/Cy7. 2.3. flat top bbq grills propaneWebThe EVOS Light Cube, Cy5 2.0, is ideal for use with Alexa Fluor 647, Cy5, DRAQ 5, NucRed Live 647, SYTO 60, and other fluorophores with excitation and emission maxima near 635 and 692 nm. The EVOS Light Cube, Cy5 2.0, offers you these important advantages: • Uniform illumination across the entire field of view for superb image quality flat top bbq mitre 10

"WebCy5 (Cyanine-5) is a fluorescent compound with an excitation peak at 651 nm and an emission peak at 670 nm. It can be excited using a 640 nm laser paired with a 670/30 nm bandpass filter, a configuration that can be found, for … " - Cy5 thermo

Cy5 thermo

Goat anti-Rat IgG (H+L) Cross-Adsorbed, Cyanine5 (A10525)

WebRabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A10523) in ICC/IF Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, Cy5 was performed using A-431 cells stained with EGFR (EP38Y) Rabbit Monoclonal Primary Antibody ( Product # MA5-14485 ). WebExplore our services and support solutions at thermofisher.com/instrumentservices. Specifications Performance specifications System specifications Dedicated computer requirements Optical configuration Resources Product Brochure: QuantStudio AbsoluteQ Digital PCR System Infographic: Comparing real-time and digital PCR technologies

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WebCy5 Contents & Storage Contains 1 vial of streptavidin, Cy5 conjugate. Store in refrigerator (2–8°C). Figures Flow cytometry histogram showing human mononuclear cells incubated with anti-CD4 biotin and followed by staining with Cy5 streptavidin. Customers who viewed this item also viewed Streptavidin, Alexa Fluor™ 647 conjugate WebTexas Red (and Texas Red-X) Dye. Invitrogen Texas Red dye is a bright red-fluorescent dye with excitation ideally suited to the 561 or 594 nm laser lines. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Texas Red sulfonyl chloride is susceptible to hydrolysis and its low solubility in water may complicate ...

WebApr 12, 2024 · The protein concentrations of mEVs and mEV-depleted samples were measured using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific, USA). The complete isolation protocol of mEVs has been submitted to the EV-TRACK (ID: EV200018) . ... Twelve hours after oral administration of Cy5-labeled mEVs, colons were collected … WebThermo Scientific™ QuantStudio™ 5 Spectral Calibration Plate (Cy™5.5 dye), 96-well, 0.1ml Catalog number: A45218 Related applications: Food & Beverage Microbiology Technical Support Customer Service This plate establishes the pure dye spectra value for the Cy™5.5 dye on Applied Biosystems™ QuantStudio™ 5 96-well 0.1-mL real-time …

WebThermo Fisher Scientific: Cat#SH3025602: Penicillin-Streptomycin (10,000 U/mL) Thermo Fisher Scientific: Cat#15140122: RPMI 1640, 1× without L-glutamine: Thermo Fisher Scientific: Cat#MT15040CV: Sodium azide: Sigma-Aldrich: Cat#S2002: Sodium hydroxide, 1.0 N Standardized Solution: Thermo Fisher Scientific: Cat#AA35629K2: Trypan Blue … Web1 day ago · Pretreat 3 was used for 30 min and the fluorescent probes used included TSA-FITC, TSA-Cy3 and TSA-Cy5 (Perkin Elmer, NEL744E001KT and NEL754001KT), samples were mounted in ProLong Gold (Thermo ...

WebInvitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes …

WebJun 25, 2012 · Cy5 was conjugated to the 5′-end of MT01, and then dissolved with PBS. Cells were cultured on 24 × 24 mm coverslips at a density of 1 × 10 4 cells/well overnight. Then, cells were treated with 1 μg/mL Cy5-labeled MT01 for 3, 6 and 12 h. Cells were washed three times with PBS according to the time points. flat-top beamWebJul 12, 2024 · PF14-SCO-Cy5 complexes were not targeted to acidic organelles during the first hour of internalisation, suggesting that mostly macropinocytosis and caveolae-mediated endocytosis, i.e., the cellular uptake pathways typically triggered by PF14, ... (Thermo Fisher Scientific, Waltham, MA, USA) were washed with 0.1% Tween-20 in TBS before ... flat top beam opticsWebThermo Fisher Scientific은 수십 년의 경험과 독자적인 어세이 설계 알고리즘 및 습식 실험실 검증 프로세스를 사용하여 동일한 ... 표적 3(ABY), 표적 4(CY5)를 검출합니다. 이 그림은 MAP16 플레이트의 단일 반응에서 QuantStudio Absolute Q … cheddar cheese wraps flat top bbq stainless steelWebHigh sensitivity and wide dynamic range. Applied Biosystems QuantStudio Real-Time PCR systems can detect very small changes, as low as 1.5 fold, in gene expression, that may be missed by other systems. A dynamic range of up to 10 logs tracks broad changes in expression between different types of samples and conditions. cheddar cheese yeast breadWeb442. 505. PerCP. 488. 675. Notes: Acridine Orange, longpass emission. To visualize Acridine Orange in green and orange/red emission wavelengths, we recommend using this light cube with the EVOS FL color or EVOS FL Auto cell imaging systems. The AO cube passes simultaneous green, yellow, and red fluorescence, which requires color camera … cheddar cheese woolworthsWebInvitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes … flat-top beam shaper