WebApr 12, 2024 · Cell proliferation assay A cell proliferation assay was conducted using the Cell Counting Kit-8 (CCK-8; Fude Biological Technology Co., China). After 48 h of … WebThe Cell Counting Kit-8 (CCK8) is a one-bottle kit that requires no premixing. This non-radioactive, sensitive colorimetric assay allows accurate live cell counting in a cell proliferation or cytotoxicity assay applications. CCK8 based assay has excellent correlation with results of traditional radioactive [ 3 H]-thymidine incorporation assay.
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WebFlow cytometry was used to analyze cell cycle progression and apoptosis. EdU and CCK-8 assays were employed to detect DNA synthesis and cell proliferation, respectively. After … WebThe ability of cell proliferation, migration and invasion was measured using Cell Counting Kit-8 (CCK-8) and wound-healing and transwell invasion assays. The protein of AURKB, …
WebApr 6, 2024 · Mitochondria are essential organelles in balancing oxidative stress and cell death during cancer cell proliferation. Rapid tumor growth induces tremendous stress on mitochondria. The mammalian tumor necrosis factor-α-induced protein 8-likes (TIPEs) family plays critical roles in balancing cancer cell death and survival. WebSep 22, 2024 · The CCK8, EdU and cell colony formation assays were used to observe the effects of ARCSP on cell proliferation. Our results indicated that ARCSP significantly …
WebJul 6, 2024 · NCSTN promotes HCC cell growth and metastasis in vitro. a The effects of NCSTN knockdown and overexpression were examined by western blotting analysis in HCCLM3 and Hep3B cells. Loading control was assessed by β-actin. b CCK8 assays showed NCSTN depletion inhibited cell growth of HCCLM3 and NCSTN overexpression … WebApr 11, 2024 · In addition to affecting cell growth, MPs can induce tissue damage and inhibit tissue development. ... 24, and 36 h using the Cell counting kit-8 reagent (Dojindo Laboratories, Kumamoto, Japan). iMARK TM Microplate Absorbance Reader (Bio-Rad, California, USA) was used for obtaining the absorbance value at 450 nm. EdU: Cells …
WebCells were treated with 17β-estradiol (E2), and cell proliferation was determined using the cell counting kit-8 assay. Using siRNA, ER-β was downregulated in RCC cells and the …
WebMar 6, 2024 · 2.3 Cell Counting Kit-8 (CCK8) assay Mitochondrial membrane potential was examined with a CCK-8 kit (MCE, Shanghai, China) according to the manufacturer’s instructions. Briefly, 10 µL of CCK-8 solution was added to … thai wyckoff njWebApr 14, 2024 · Background In mouse, it was discovered that resveratrol (Res) enhanced osteoporosis (OP) by boosting osteogenesis. Besides, Res can also have an impact on MC3T3-E1 cells, which are crucial for the control of osteogenesis and thus increase osteogenesis. Although some articles have discovered that Res enhanced autophagy to … synonyms for resistedWebAug 1, 2024 · Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8; Dojindo, Japan). Briefly, 5 × 103 cells of HCT116 were plated in 96 well plates and were incubated with either 0.1% (v/v) DMSO (control) or various concentrations (0, 1, 10, 20, 40, 80, 120, 160 μM) of Apigenin and 5-Fu (0, 1, 5, 10, 20, 50, 100, 200 μM) for 24 h at 37 °C. synonyms for restlesslyWebJun 9, 2015 · Cells were treated with TSIIA and infected with 14-3-3ζ-overexpressing adenovirus, the expression level of 14-3-3ζ was determined by western blotting (WB), cell viability was detected by Cell Counting Kit-8 (CCK8), cell invasion and migration was evaluated by transwell assay, and cell apoptosis was detected by flow cytometry. Results synonyms for restlessnessWebProduct Information. Cell Counting Kit-8, based on WST-8 (chemical name: 2- (2-methoxy-4-nitrophenyl)-3- (4-nitrophenyl)-5- (2,4-disulfobenzene)- 2H-tetrazole monosodium salt), is a rapid and highlysensitive detection kit widely used in cell proliferation and cytotoxicity.In the presence of electron coupling reagent, WST-8 can be reduced by ... thai wynnumWebApr 13, 2024 · Cell growth was determined by CCK8. c and d, A Colony-formation assay was performed to determine the oncogenic growth of CaSki and HeLa cells. ... searching for molecular regulators of tumor cell growth has been an important goal of cervical cancer research. TMEM family proteins can span the entire lipid bilayer and anchor to the … synonyms for resolutelyWebFeb 6, 2024 · CCK8 Assay. Cell growth was measured using the cell proliferation reagent WST-8 (Roche Biochemicals, Mannheim, Germany). After plating cells in 96-well microtiter plates (Corning Costar, Corning, NY) at 1.0× 10 3 /well, 10 μL of CCK8 was added to … www.ncbi.nlm.nih.gov thai wyoming